English
聯系我們
網站地圖
郵箱
舊版回顧



科學家繪制造血干細胞擴增組織的3D轉錄組圖譜

文章來源:動物研究所   發布時間:2019-05-10  【字號:     】  

  血液系統中貯藏著一種具有自我更新、分化成各種血細胞潛能的成體干細胞,稱為造血干細胞,它能夠維持機體長久造血和組織穩態。造血干細胞移植是惡性血癌的有效治療手段,但干細胞來源不足成為限制該治療廣泛應用的瓶頸。因此,造血干細胞的發育,尤其是造血干細胞擴增的研究備受關注。然而,現在的研究主要集中在特定細胞群體或關鍵因子的“線性化”或“平面化”解析,缺乏全面“立體化”的分析。從系統生物學角度解析擴增性造血器官,將為造血干細胞擴增的機制研究提供更多的啟示。鑒于造血發育過程的高度保守性,中國科學院動物研究所劉峰研究組及合作者以斑馬魚尾部造血組織(在功能上類似于哺乳動物的胎肝,為造血干細胞擴增及分化提供了特定的微環境)為研究對象,利用多重轉錄組測序的方法,繪制了擴增性造血器官的3D轉錄組圖譜,闡釋了造血干細胞和微環境細胞之間的分子調控網絡。

  首先,為了系統性地研究斑馬魚尾部造血組織如何支持造血干細胞擴增、分化的機制,劉峰實驗室利用三種轉錄組測序方法,特定類型群體細胞轉錄組、空間轉錄組和單細胞轉錄組,詳細解析了斑馬魚尾部造血組織的時空特性。不同發育時期特定細胞群體的轉錄組測序分析表明,造血干細胞與相應微環境細胞具有協同發育和共享基因表達的特點。空間轉錄組測序反映細胞位置信息,揭示了多種微環境因素對造血干細胞的不同調控方式,并實現了三維轉錄組重構斑馬魚造血器官。單細胞轉錄組數據更精細闡釋尾部造血組織細胞類群、細胞特征及細胞間分子調控,并發現造血干祖細胞分化和細胞周期存在一定的關聯性。多重轉錄組測序綜合分析揭示了造血干細胞在尾部造血組織的擴增受到內源性和外源性信號的共同調控,鑒定了新的造血干細胞的標記基因,發現了影響造血干細胞擴增的信號通路,完善了造血干細胞-微環境細胞間分子調控網絡。例如,染色質結構維持蛋白smchd1,通過調節造血干細胞增殖促進其擴增;微環境細胞來源的Integrin 信號通路負向調控造血干細胞擴增。同時此項工作還構建了斑馬魚尾部造血組織多重轉錄組數據庫(http://www.picb.ac.cn/hanlab/ichtatlas/Home),可以檢索任何基因在造血組織的表達譜,包括基因表達的時間、細胞類型及相對空間位置。

  該工作不僅發現了造血干細胞特征基因、細胞狀態與細胞分化的關系,闡釋了微環境和造血干細胞互作的調控網絡,更為血液系統研究提供了豐富的數據資源;同時,也將為造血干細胞的體外擴增和造血類器官的體外組裝提供理論指導。該研究論文“A 3D atlas of hematopoietic stem and progenitor cell expansion by multi-dimensional RNA-seq analysis430日在線發表于Cell Reports

  動物所博士研究生薛媛媛和中科院計算生物學研究所博士研究生劉登輝為文章共同第一作者,動物所研究員劉峰和計算生物學所研究員韓敬東為文章共同通訊作者。中科院生物化學與細胞生物學研究所研究員景乃禾與中科院廣州生物醫藥與健康研究院研究員彭廣敦在空間轉錄組測序方面提供了大力支持。該研究得到國家自然科學基金委、國家重點基礎研究發展計劃和中科院器官重建與制造戰略性先導科技專項的資助。

 

  圖1:多重轉錄組測序解析斑馬魚造血器官。多重轉錄組測序,包括:特定類型群體細胞轉錄組測序、空間轉錄組測序和單細胞轉錄組測序,揭示斑馬魚尾部造血組織的時空特性。

 

圖2:斑馬魚造血器官轉錄組數據庫 




(責任編輯:葉瑞優)

附件:

專題推薦

相關新聞


© 1996 - 中國科學院 版權所有 京ICP備05002857號  京公網安備110402500047號  聯系我們

地址:北京市三里河路52號 郵編:100864

C9999www.318548.com www.if03.com | www.567999.cc | www.920273.com | 868124.com | www.xz4.cc | www.3377.cm | www.767888.cc | 500彩票www.175904.com | www.3193.net | www.5868.cn | www.194622.com | www.345303.cc | www.772776.com | www.864585.com | 676366.com | www.fw00.com | www.2570.net | www.61301.com | www.810906.com | www.9076.me | www.578352.com | 587845.com | 855492.com | www.no57.com | www.39731.cc | www.108744.com | www.284186.com | www.534674.com | www.863676.com | www.37zw.cc | www.60452.com | www.658990.com | 573014.com | www.1wx.cc | www.070293.com | www.877747.com | www.nr8.com | www.56pb.com | www.8552.in | www.065865.com | www.612687.com | 930883.com | www.ts78.com | www.71qb.com | www.8170.vip | www.025979.com | 27791.com | 488985.com | www.iw37.com | www.cp0268.com | www.144281.com | www.599072.com | 945002.com | 68彩票www.68682q.com | www.17989.com | www.017744.com | www.057207.com | www.55418.cc | www.163828.com | www.285921.com | www.387408.com | www.828176.com | 653760.com | www.nq62.com | www.2425.in | www.9595.tv | www.795608.com | 113901.com | www.466036.com | www.28do.com | www.2172.win | www.168828.com | www.490026.com | www.948607.com | 737449.com | 623925.com | 281847.com | www.977581.com | www.819193.com | www.875413.com | 大赢家彩票平台www.702392.com | 大玩家彩票www.717224.com | 880816.com | 972846.com | www.887700.com | 541678.com | www.947675.com | www.968397.com | www.778389.com | www.mu96.com | www.78320.com | www.623386.com | www.954809.com | www.991048.com | www.018888.com | js3311.com | 289671.com | www.ry9.cc | www.6793.biz | www.84999.cc | www.105325.com | www.287215.com | www.612392.com | 500万彩票www.96386l.com | 874300.com | www.6340.cn | www.82799.cc | www.308543.com | www.499636.com | www.791092.com | www.gj48.com | www.6496.vip | www.16508.com | www.118044.com | www.444307.com | 185617.com | www.35332.cc | www.215514.com | www.362302.com | www.cb10.com | 150126.com | www.720665.com | www.374566.com | 99彩票www.223268.com | 13e彩票www.809387.com | www.pl03.com | www.306.in | www.118721.com | www.280521.com | www.452618.com | www.562587.com | www.881540.com | 739867.com | www.173961.com | www.f17.cc | www.24997.com | www.22555.cc | www.19251.com | www.329882.com | www.621014.com | www.760196.com | www.787342.com | www.707686.com | www.662929.com | www.750422.com | www.961465.com | www.863062.com | www.930098.com | 539062.com | 金冠彩票www.c4528.com | www.id26.com | www.hm68.com | www.45po.com | www.2650.pw | www.266092.com | www.663911.com | www.967191.com | www.997402.com | 59554.com | www.ni13.com | www.979549.com | www.275192.com | 253295.com | www.33ds.com | www.3917.bid | www.41481.com | www.90047.com | www.024798.com | www.022288.cc | www.141403.com | www.390128.com | www.730476.com | www.781009.com | 福彩www.26878j.com | www.07ag.com | www.1080.site | www.55557.cc | www.059186.com | www.244443.cc | www.949821.com | www.981892.com | www.950841.com | www.bt26.com | www.44148.cc | www.095278.com | www.9936.in | www.7583.cc | www.55323.com | www.166122.cc | www.536513.com | www.787966.com | 668876.com | 949215.com | 8330.biz | www.984682.com | www.945850.com | 吉利彩票www.80065y.com | www.cp5669.com | www.kr63.com | www.m21.hk | www.58158.com | www.317665.com | www.704228.com | 679201.com | 947270.com | 659904.com | www.id18.com | www.70io.com | www.0491.xyz | www.121160.com | www.720798.com | www.015734.com | www.yi49.com | www.950445.com | www.195873.com | www.k54.com | www.922523.com | www.172885.com | www.469.pw | jb38.com | www.158772.com | www.603705.com | www.542658.com | www.556685.com | www.586289.com | www.493228.com | www.699202.com | 871728.com | 221447.com | 379653.com | www.736375.com | www.xy8.com | www.cai2133.com | www.tg5.com | www.19nr.com | www.7319.com | www.2901.vip | www.79956.cc | www.086062.com | www.724518.com | www.761511.com | www.350.cm | www.9224.cc | www.062472.com | www.179973.com | www.263120.com | www.393005.com | www.691507.com | www.898897.com | www.921071.com | www.888568.cc | gv25.com | 271226.com | www.hw15.com | www.63by.com | www.903746.com | 443636.com | 622605.com | 天下彩www.308680.com | www.4sg.com | www.29vq.com | www.3584.biz | www.92998.com | www.467542.com | www.333010.com | www.950753.com | www.989881.com | 053160.com | www.7dt.com | www.99sq.cc | www.12318.cc | www.39066.cc | www.396700.com | www.756018.com | 971425.com | 271332.com | 439287.com | 彩票5www.550419.com | www.lq19.com | www.16ib.com | www.1814.vip | www.084735.com | www.677025.com | www.713839.com | www.922255.com | www.947868.com | 358975.com | 234047.com | 271236.com | www.228318.com | www.664881.com | 杏彩www.153328.com | www.jl67.com | www.79oh.com | www.11113.com | www.53552.com | www.305283.com | www.470955.com | www.641230.com | 165293.com | 098951.com | 871452.com--www.dingji821.com | 318670.com | bm40.com | www.96qz.com | www.048170.com | www.781225.com | www.979482.com | 647478.com | 029625.com | www.29yv.com | www.89rf.com | www.096.date | www.024930.com | www.317797.com | www.292969.com | www.173505.com | www.575570.com | www.675161.com | 418904.com | 586742.com | www.021405.com | www.vx57.com | www.nr00.com | www.127971.com | www.178165.com | www.339053.com | www.388805.cc | www.822879.com | 951464.com | 949946.com | 652088.cc | 761574.com | www.33ch.com | www.0948.net | www.76621.cc | www.106668.com | www.169786.com | www.018433.com | www.70ep.com | www.51297.cc | www.008941.com | www.358554.com | www.839337.com | 707651.com | www.32008.com | www.38429.cc | www.257144.com | www.374056.com | www.600540.com | www.700247.com | www.207551.com | www.982563.com | www.862893.com | www.980710.com | www.897712.com | www.891105.com | www.798555.com | www.735185.com | www.644410.com | www.840059.com | 158902.com | 017153.com | 971145.com | 天天彩票www.2373d.com | www.nc88.cc | www.63ss.com | www.136.date | www.495.tv | www.44546.com | www.26629.cc | www.7896.org | www.11615.com | www.51561.com | www.101606.com | www.100065.com | www.192666.com | www.090768.com | www.691908.com | www.925605.com | www.913665.com | 443504.com | 好彩头www.51331j.com | www.cb45.com | www.20qr.com | www.27eo.com | www.71482.com | www.232040.com | www.155825.com | www.454526.com | www.762305.com | www.751720.com | www.982551.com | www.yp89.com | www.49rr.com | www.c91.in | www.2wn.cc | www.76.gg | www.84ta.com | www.35xp.com | www.431.org | www.1474.vip | www.59811.cc | www.127638.com | www.180033.com | www.569536.com | www.911807.com | www.889788.com | www.840733.com | 147827.com | 3072.biz | 478082.com | www.996547.com | www.995868.com | 595350.com | www.970130.com | www.905505.com | www.848549.com | 092391.com | 744618.com | www.996835.com | www.972548.com | www.907368.com | www.968110.com | www.919124.com | www.837847.com | www.990337.com | www.928844.com | www.931150.com | www.796708.com | www.769885.com | www.711381.com | 692250.com | 4623.biz | www.ag46.com | 9号彩票www.80767c.com | 009084.com | 网易彩票www.076wy.com | 844417.com | 513757.com | 6078.biz | 752803.com | www.pp9999.com | 128724.com | 778369.com | www.097.bid | 647531.com | 659330.com | www.505447.com | 七星彩票www.44qxc.com | 639924.com | www.np2.cc | 380860.com | 037713.com | www.499628.com | 925404.com | 663876.com | 华夏彩票www.hx1165.com | 544212.com | 654969.com | 737107.com | 365423.com | 945700.com | 60uz.com | 231621.com | 139792.com | www.987052.com | www.928535.com | www.666661.cc | www.568746.com | www.558221.com | www.683758.com | www.448577.com | www.178534.com | www.054068.com | www.13086.com | www.q80.in | 545564.com | 930287.com | www.789227.com | www.72oj.com | 902074.com | www.331760.com | www.wd55.com | 936912.com | www.859916.com | www.795926.com | www.581783.com | www.388470.com | www.47006.com | www.020813.com | www.502810.com | 861154.com | 402437.com | cp606.com | 005761.com | www.994374.com | www.914464.com | www.809798.com | www.926323.com | www.900083.cc | www.875819.com | www.793848.com | www.741163.com | www.716646.com | www.751901.com | www.708396.com | www.761420.com | www.683911.com | www.581314.com | www.463778.com | www.388639.com | www.611382.com | www.753905.com | www.896013.com | www.810495.com | 089025.com | 076484.com | 576624.com | 850769.com | 569vn.com | 957498.com | 80852.com | 090277.com | 471883.com | 504046.com | 984494.com | www.886328.com | www.281422.com | www.xr70.com | 043281.com | www.727258.com | www.785761.com | www.382813.com | www.84439.com | 凤凰彩票www.66652m.com | 558036.com | www.201933.cc | www.18oc.com | www.a49.top | www.n65.net | www.a04.cc | www.27568.com | www.42525.com | www.50068.cc | www.073605.com | www.036740.com | www.253504.com | www.447702.com | www.590499.com | www.299671.com | www.92236.com | 718332.com | www.747762.com | www.281048.com | 8号彩票www.81520c.com | www.77zx.com | www.23ql.com | www.zb13.com | www.cy80.com | www.bs71.com | 63lt.com | www.vn22.com | www.2102.vip | www.1485.com | www.500.cc | www.230.live | www.98fi.com | www.8011.loan | www.7716.tv | www.0781.xyz | www.7544.cc | www.33038.cc | www.345119.com | www.260738.com | www.216179.com | www.130457.com | www.054985.com | www.80388.com | www.53959.com | 990853.com | www.52nu.cc | www.r65.com | www.nj85.com | www.ra02.com | 天天乐彩票www.281380.com | 大赢家彩票www.529411.com | www.ne86.com | www.at31.com | www.4838.biz | www.141.in | www.7333.live | www.1903.vip | www.3981.xyz | www.88337.cc | www.062784.com | www.019025.com | www.265432.cc | www.200013.cc | www.541627.com | www.531229.com | www.415959.com | www.659083.com | www.840844.com | 083972.com | www.973417.com | www.850398.com | 121571.com | www.69756.com | www.355436.com | www.181878.com | www.582038.com | www.390116.com | 737811.com | www.886353.com | 500彩票网www.61655x.com | 821257.com | 626543.com | www.982651.com | www.752725.com | www.536757.com | www.293389.com | www.288818.com | www.520504.com | www.525917.com | www.280329.com | www.066982.com | www.28515.cc | www.68jc.com | 亿发彩票www.870933.com | cc-betwin100.com | www.954962.com | 741128.com | www.980763.com | www.731210.com | www.475315.com | www.404849.com | www.166112.cc | www.113111.com | www.112909.com | www.36763.cc | www.69os.com | www.cai2424.com | 732531.com | www.964489.com | www.770034.com | www.520473.com | www.88867.com | www.198974.com | www.943767.com | www.760084.com | www.843823.com | www.967772.com | www.897712.com | www.739453.com | www.625873.com | www.656613.com | www.632452.com | www.683839.com | www.867503.com | www.886132.com | www.25176.cc | www.198333.com | www.110967.com | www.62897.cc | www.038397.com | www.090522.com | www.66782.cc | www.34478.com | www.922.date | www.60421.com | www.205793.com | www.85535.cc | www.22575.cc | www.87ex.com | www.6987.in | www.8sl.com | www.318529.com | www.518980.com | www.407027.com | www.947411.com | www.796409.com | www.670113.com | www.933582.com | www.968561.com | www.779819.com | www.820325.com | www.688124.com | www.447365.com | www.449721.com | www.390829.com | www.317354.com | www.220811.com | www.161712.com | www.385133.com | www.363438.com | www.382892.com | www.508322.com | www.304789.com | www.007422.com | www.98761.cc | www.536099.com | www.08493.com | www.962847.com | www.988720.com | www.949638.com | www.752114.com | www.645501.com | www.686109.com | 493337.com | 172172.com | 372825.com | www.976125.com | www.830884.com | www.871717.com | www.778035.com | www.740784.com | www.602538.com | www.550223.com | www.625708.com | www.891841.com | www.764448.com | www.617782.com | www.643939.com | www.950993.com | 爱彩乐www.www.ac9911.com | 192387.com | 898745.com | www.81669.com | www.104082.com | www.042969.com | www.51326.cc | www.21876.com | www.026263.com | www.66025.cc | www.019142.com | www.95363.com | www.118717.com | www.253872.com | www.219948.com | www.385064.com | www.261068.com | www.370028.com | www.581129.com | www.589050.com | 85122.com | www.971348.com | www.737496.com | www.833400.com | www.740247.com | www.622763.com | 324214.com | www.830044.com | www.966993.com | www.860693.com | www.870814.com | www.961829.com | www.812627.com | www.663600.cc | www.859849.com | www.569179.com | www.380523.com | www.372131.com | www.286861.com | www.111286.com | www.254593.com | www.382089.com | www.529455.com | www.606069.com | www.605432.com | www.462299.com | www.355014.com | www.526365.cc | www.441111.com | www.288052.com | www.212154.com | www.192730.com | www.108222.com | www.76769.com | www.260167.com | www.320871.com | www.548399.com | www.405239.com | www.337090.com | www.173726.com | www.298141.com | www.201175.com | www.099991.com | www.238538.com | www.055431.com | www.28761.cc | www.239501.com | www.948385.com | www.796143.com | www.989233.com | www.711986.com | www.955954.com | www.835982.com | www.727923.com | www.596675.com | www.819570.com | www.566525.com | www.700047.com | www.657814.com | www.655656.com | www.751632.com | www.623719.com | www.588479.com | www.689702.com | www.456588.com | www.113814.com | www.40836.com | www.07900.cc | www.0402.org | www.43li.com | www.24qn.com | www.31jp.com | www.39353.cc | www.9224.com | www.638.website | www.4737.xyz | www.3yn.com | www.83al.com | www.72720.cc | www.84456.com | www.22319.com | www.9877.me | www.0060.com | www.xu64.com | www.cp7376.com | 311819.com | 44466.com | 098822.com | www.875909.com | www.851252.com | www.669295.com | www.433033.com | www.293653.com | www.038808.com | www.013422.com | www.150159.com | www.263717.com | www.190260.com | www.030086.com | www.287167.com | www.292932.com | www.566656.cc | www.371821.com | www.363731.com | www.171843.com | www.069986.com | www.78187.com | www.610136.com | www.842826.com | 304148.com | 170404.com | 946864.com | www.984812.com | www.851113.com | www.yt03.com | 166459.com | 爱购彩票www.3552z.com | 012132.com | www.981391.com | www.776796.com | www.764346.com | 978854.com | 033827.com | 1504.cc | 661409.com | www.816511.com | www.800976.com | www.119616.com | www.023333.com | www.28054.com | www.076728.com | www.80310.com | www.185276.com | www.382870.com | www.446605.com | www.532680.com | www.232221.com | 170803.com | 199093.com | 381178.com | www.973605.com | www.836227.com | www.619206.com | www.764606.com | www.591022.com | www.369429.com | www.359286.com | www.247244.com | www.049055.com | www.17989.com | 22334.cc | www.850923.com | www.748666.com | www.568093.com | www.872877.com | www.982323.com | www.751430.com | www.571130.com | www.666425.com | www.35153.cc | 彩神帝www.368200.com | www.947507.com | www.579808.com | www.8319.top | www.er02.com | www.974871.com | www.84460.com | www.045542.com | www.2054.vip | www.512652.com | www.237086.com | www.64088.com | www.029651.com | www.028421.com | www.108886.com | www.779685.com | 345734.com | 036643.com | www.756932.com | www.521219.com | www.286411.com | www.233338.com | 004121.com | www.749474.com | www.155323.com | www.433530.com | www.545645.com | www.174741.com | www.939513.com | 56755.com | 412097.com | 6323.biz | 760097.com | 460021.com | www.844418.com | www.829640.com | www.717081.com | www.901884.com | 3077.biz | www.766694.com | www.485793.com | www.173659.com | www.260345.com | www.191591.com | www.062252.com | www.7598.me | 彩61www.097968.com | 496862.com | 82ie.com | 423711.com | www.781597.com | 179961.com | 155009.cc | www.846479.com | www.930692.com | www.746726.com | 994503.com | www.843722.com | www.926852.com | www.600067.cc | www.323251.com | www.211277.com | www.94417.com | www.19oq.com | 02ne.com | 499715.com | www.927906.com | www.93ed.com | 618138.com | www.66872.cc | 182491.com | www.982234.com | 216554.com | 953414.com | www.787333.com | www.997410.com | www.821496.com | www.338561.com | www.289212.com | www.29836.cc | www.dh07.com | www.cai7166.com | 968447.com | 033972.com | 262234.com | www.911855.com | www.669431.com | www.747866.com | 718635.com | 358534.com | www.921259.com | www.926003.com | www.620935.com | www.604971.com | www.309749.com | www.120469.com | www.7878.vip | www.js54.cc | www.58gg.com | www.64pl.com | www.cp441.com | www.fw66.cc | www.wh99.com | 00309.com | 108473.com | 4690.biz | www.862065.com | www.905195.com | www.873624.com | www.810506.com | www.433345.com | www.247232.com | www.057744.com | www.98644.cc | www.1825.com | www.mj91.com | www.p24.biz | www.22uj.com | 637036.com | 882018.com | www.927502.com | www.719570.com | www.376323.com | www.481177.com | www.268238.com | www.320813.com | www.292576.com | www.089917.com | www.210055.com | www.159965.com | www.438559.com | www.305902.com | www.076916.com | www.33622.com | www.63395.cc | www.062785.com | www.279471.com | www.443143.com | www.216674.com | www.085700.com | www.351.tv | www.0837.bid | 吉利彩票www.80075m.com | 414811.com | www.775876.com | www.715796.com | www.377085.com | www.288762.com | www.79317.com | www.30675.com | www.36604.com | www.2167.xyz | www.33ex.com | 9492.me | 731845.com | 664548.com | 275976.com | www.730167.com | www.510101.com | www.301896.com | www.286387.com | www.028168.com | www.40402.cc | www.55dv.com | www.fo5.cc | www.cai1232.com | www.997858.com | www.555914.com | www.200988.com | www.5869.vip | www.052151.com | www.31cc.com | 21022.cc | www.805615.com | www.379789.com | www.270065.com | www.26528.com | www.xg23.com | 4897.biz | www.655772.com | www.300075.cc | www.203968.com | www.563530.com | www.633566.com | www.379585.com | www.298908.com | www.101464.com | www.15913.com | www.s00.top | pk46.com | 01qd.com | 526255.com | www.788544.com | www.789214.com | www.862165.com | www.844518.com | www.885368.com | www.669749.com | www.384365.com | www.360105.com | www.453656.com | www.272513.com | www.033976.com | www.185288.com | www.059666.com | www.183012.com | www.545412.com | www.761811.com | www.872085.com | www.729242.com | www.790048.com | www.719871.com | www.511790.com | www.229663.com | www.28he.com | www.996826.com | www.289292.com | 35934.com--35934.com | www.983722.com | www.698415.com | www.40637.com | www.28fh.com | 971476.com | www.949638.com | www.517095.com | www.258336.com | www.110291.com | www.5811.in | 328789.com | 526059.com | 579260.com | www.874587.com | www.707385.com | www.586694.com | www.314445.com | www.370044.com | www.394422.com | www.085891.com | www.66117.cc | www.0353.date | www.70600.com | www.015443.com | www.4kn.com | 357172.com | www.954018.com | www.60407.com | www.98186.cc | www.1454.in | www.31xm.com | 185192.com | 893071.com | www.949267.com | www.660095.com | www.712244.com | www.653551.com | www.551110.com | www.254267.com | www.092343.com | www.141080.com | www.155387.com | www.169921.com | www.070180.com | www.008672.com | www.37683.cc | www.8ha.cc | 875.club | www.08377.com | www.2104.cn | www.2829.biz | www.7478.vip | 乐彩网www.3416m.com | 951334.com | 964142.com | www.907178.com | www.582128.com | www.700498.com | www.367125.com | www.437001.com | www.385533.com | www.220827.com | www.38196.cc | www.zn91.com | 715019.com | www.097999.com | www.16455.com | 490225.com | www.588786.com | www.262859.com | www.p47.net | www.380163.com | www.092263.com | 620527.com | www.553171.com | www.5138.cm | 559092.com | www.668274.com | www.413716.com | www.607630.com | 2078.com | www.270179.com | www.111348.com | www.802735.com | www.586664.com | www.240289.com | www.655151.com | www.485900.com | www.231570.com | www.182865.com | www.103622.com | www.64nx.com | www.av55.com | 800228.com | www.750010.com | www.603292.com | www.365334.cc | www.201045.com | www.26596.com | www.5334.org | www.iv88.cc | www.nk28.com | 720036.com | 13678.com | www.909209.com | www.537848.com | 778072.com | www.793203.com | www.676210.com | www.287661.com | www.089094.com | www.8100.biz | www.th95.com | 七天彩www.00665e.com | 易盈彩票www.7793f.com | 664447.com | www.726355.com | www.521777.com | www.133131.com | www.302661.cc | www.089046.com | www.3262.com | www.mt1.com | 网易彩票www.36788s.com | 775202.com | www.751797.com | www.711010.com | www.345303.cc | www.083234.com | www.1434.cm | www.ol78.com | www.28gc.com | www.839046.com | 953584.com | www.963918.com | www.831125.com | www.362686.com | www.263830.com | www.929953.com | www.579405.com | www.48891.com | 幸运彩票www.luck887.com | www.651538.com | www.243822.com | www.080288.com | www.23468.com | www.86335.com | www.9026.me | www.3ve.com | 039992.com | 978513.com | www.432501.com | www.968.cm | www.220278.com | 44416.com | www.783990.com | www.408578.com | www.444151.com | www.630280.com | www.592785.com | www.230407.com | www.36855.cc | www.o21.cn | 365972.cc | www.tv47.com | 553051.com | www.876252.com | www.927812.com | www.610225.com | www.525098.com | www.705068.com | www.302915.com | www.018157.com | www.877.mobi | www.ax11.com | 173912.com | www.973263.com | 127191.com | 026853.com | www.21es.com | 657490.com | www.970987.com | www.557994.com | www.933982.com | www.951090.com | www.588748.com | www.212337.com | www.129776.com | www.52953.cc | www.9953.in | www.0zs.cc | 731364.com | www.967401.com | www.593295.com | www.369034.com | www.797526.com | www.822042.com | www.368892.com | www.172313.com | www.44474.cc | www.74581.com | www.334388.com | www.200965.com | www.959.in | www.ow63.com | 360176.com | www.567587.com | www.784303.com | www.533570.com | www.428701.com | www.038831.com | www.ja35.com | 3762.biz | www.955689.com | www.936391.com | www.570618.com | www.136391.com | www.35081.com | www.kg79.com | 71193.com | www.81002.com | www.968469.com | www.587957.com | www.454057.com | www.451322.com | www.175381.com | www.108475.com | www.89370.com | www.25oq.com | 521565.com | 610783.com | www.729965.com | www.362110.com | www.521585.com | www.780298.com | www.529139.com | www.068102.com | www.117010.com | www.9679.com | 456147.com | www.980069.com | www.570278.com | www.186070.com | www.318552.com | www.019368.com | www.ig86.com | www.cai911.com | www.bh14.com | mn40.com | www.805401.com | www.937200.com | www.526368.com | www.518565.com | www.365756.cc | www.267213.com | www.068170.com | www.88yh.cc | 5495.com | www.955462.com | www.561097.com | www.226747.com | www.08980.com | www.12bv.com | 367569.com | www.861277.com | www.555492.com | www.660403.com | www.885785.com | www.771490.com | www.817363.com | www.607701.com | www.575690.com | www.691911.com | www.579734.com | www.899826.com | www.511889.com | www.350435.com | www.0133.wang | 396802.com | www.857713.com | www.337195.com | www.84336.com | www.7ip.com | 346234.com | www.900257.com | www.388478.com | www.97522.cc | www.214866.com | www.12646.com | www.69688.com | www.wh42.com | 373687.com | www.898298.com | www.303365.com | www.7710.top | 500彩票www.937986.com | www.838792.com | 919286.com | 372585.com | www.700842.com | www.361690.com | www.155669.com | www.26805.com | www.20ty.com | 826279.com | www.981845.com | 086666.com | www.631516.com | www.452673.com | www.269666.com | www.313882.com | www.151710.com | www.212815.com | www.241233.com | www.423559.com | www.127006.com | www.003571.com | 159彩票www.159888a.com | 03888.com | www.555156.com | www.220137.com | www.129767.com | www.1369.org | 933317.com--6365668.com | www.915295.com | www.914952.com | www.729832.com | www.635930.com | www.379002.com | www.067448.com | www.29451.com | 幸运彩票www.550116.com | www.957403.com | www.488422.com | www.9081.org | 584800.com--731111.com | www.808176.com | www.877586.com | www.642059.com | www.481112.com | www.55282.com | 酷彩网www.89959.cc | www.995901.com | www.772420.com | www.476055.com | www.030531.com | www.42wp.com | 915722.com | 128844.com | 3130.biz | www.585308.com | www.224793.com | www.5261.pw | 472118.com | 720029.com | www.779548.com | www.413765.com | www.393007.com | www.482208.com | www.020491.com | www.57um.com | www.jn0.cc | www.982205.com | www.761704.com | www.699209.com | www.772373.com | www.380384.com | www.65958.cc | www.dn65.com | www.660.hk | www.651041.com | www.313190.com | www.0513.net | www.ig15.com | www.960939.com | www.947306.com | www.285971.com | www.67po.com | 167388.com | www.539050.com | www.154505.com | www.366417.com | www.036652.com | www.im94.com | 972882.com | www.558170.com | www.542898.com | www.025549.com | www.306645.com | www.170526.com | www.5616.me | www.13481.com | www.41ji.com | 555313.com | www.949427.com | www.699746.com | www.4455.tv | www.53rj.com | www.488q.com | www.903225.com | www.380158.com | www.214925.com | www.475966.cc | www.99oa.com | www.i12.club | 428678.com | www.p20.com | 网易彩票www.26163y.com | www.905539.com | www.415442.com | www.86477.cc | www.uz52.com | www.wl11.com | 910313.com | www.900989.cc | www.362771.com | www.316287.com | www.95400.com | www.31ak.com | 520787.com | www.696111.com | www.758479.com | www.588029.com | www.303698.com | www.196446.com | 058409.com | www.868325.com | www.088201.com | 797720.com | www.679922.com | www.121474.com | www.162403.com | www.791974.com | www.658663.com | www.708987.com | www.365529.cc | www.298569.com | www.071128.com | 247722.com | www.234071.com | 941006.com | www.406573.com | www.44463.cc | 500彩票www.769588.com | www.930298.com | 梦想彩票www.33112m.com | www.956826.com | www.739601.com | www.721811.com | www.126798.com | www.31rf.com | www.965649.com | www.768232.com | www.710506.com | www.514608.com | www.468318.com | www.275391.com | www.082773.com | 496705.com | www.798089.com | www.250069.com | www.351414.com | www.39998.com | www.nm88.com | 304224.com | www.640770.com | www.616087.com | www.335584.com | www.57308.com | www.42mz.com | cai7789.com | www.672356.com | www.313365.cc | www.056069.com | www.21ve.com | www.974126.com | www.498077.com | www.383985.com | www.267273.com | www.478689.com | www.96320.cc | 807720.com | 766426.com | www.060755.com | www.52562.cc | www.68689.cc | www.us06.com | 6097.biz | 86wu.com | www.819393.com | www.934133.com | www.246345.com | 759977.com | www.371636.com | www.71hb.com | www.817009.com | www.596876.com | www.0376.biz | www.920282.com | www.51830.com | www.wa58.com | www.604924.com | www.u69.cc | www.671136.com | www.2773.cm | www.986730.com | www.227376.com | www.8455.in | 726210.com | www.553850.com | www.181874.com | www.222488.com | 007111.com | www.880950.com | www.622200.com | www.6666.la | 285937.com | www.268035.com | www.2ku.com | 119374.com | www.313971.com | www.607083.com | www.97126.cc | www.023408.com | www.66bv.com | 949091.com | www.649518.com | www.573129.com | www.09118.com | 金冠彩票www.49956q.com | 841166.com | www.767073.com | www.490987.com | www.233542.com | www.068303.com | www.23qi.com | 150127.com | www.582605.com | www.351848.com | www.91437.com | www.1528.biz | www.gn66.com | 7356.biz | www.581777.com | www.021366.com | www.96213.cc | www.5681.xyz | 502850.com--568017.com | www.938184.com | www.458722.com | www.88434.cc | www.hb12.com | 025643.com | www.500757.com | www.448632.com | www.020083.com | www.qo83.com | 90920.com | www.661612.com | www.299629.com | www.119336.com | www.q77.com | 5313.biz | www.625796.com | www.258356.com | www.36125.cc | www.x50.site | 5171.biz | www.712688.com | www.174878.com | www.53112.cc | www.5051.vip | 418889.com | www.j43439.com | www.890365.com | 大富豪www.h679.com | 676946.com | 429167.com | 049513.com | www.676446.com | www.192976.com | www.2061.club | www.k17.me | 185732.com | www.566190.com | www.78829.com | www.482.date | 凤凰彩票www.77802i.com | www.877674.com | www.608106.com | www.410247.com | www.084288.com | www.606442.com | www.672375.com | www.117753.com | www.ut60.com | www.951573.com | www.230934.com | www.422.cc | 028125.com | www.557661.com | www.134034.com | 多媒体信息发布系统_56iq数字标牌软件官网 | www.4996b.com | www.675795.com | www.334345.com | www.066921.com | www.qd11.com | 945222.com | www.592088.com | www.047319.com | 乐彩网www.3416b.com | www.736301.com | www.092276.com | 彩多多www.78949s.com | j62.xyz | www.399949.com | www.115744.com | www.re72.com | www.939532.com | www.654328.com | www.186888.com | www.076.date | 979158.com | www.700557.com | www.584728.com | www.809911.com | www.217220.com | www.20779.com | www.sz3.cc | www.885381.com | www.585214.com | www.027564.com | www.21713.cc | www.8504.vip | www.th66.cc | 473100.com | www.815120.com | www.711295.com | www.292755.com | www.5370.in | 587844.com | www.676189.com | www.106759.com | www.12321.cc | 好彩网www.668566.com | www.859038.com | www.449779.com | www.254406.com | www.6785.in | 532893.com | www.790561.com | www.221365.cc | www.323593.com | www.006727.com | www.ul25.com | www.914985.com | www.709318.com | www.187378.com | www.078917.com | www.364.cn | 657026.com | www.539215.com | www.36700.com | 博乐彩票www.35918n.com | www.836211.com | www.162785.com | www.70jc.com | www.984507.com | www.280095.com | www.08593.com | www.pd27.com | www.971284.com | www.278606.com | www.85vc.com | www.986159.com | www.311753.com | www.367.tv | 707089.com | www.595599.com | www.79387.com | www.f76.com | www.932718.com | www.207602.com | www.57ue.com | www.980417.com | www.334345.com | www.7513.shop | 22711.com | www.706376.com | www.208455.com | www.1475.vip | 311819.com | www.766108.com | www.560209.com | www.91262.com | www.ix03.com | 984261.com | www.cp077.com | www.894349.com | www.308962.com | www.317369.com | www.5884.top | 930329.com | www.658668.cc | www.653862.com | www.071216.com | www.072856.com | www.sy79.cc | www.962149.com | www.454105.com | www.71575.com | www.40xg.com | www.898595.com | www.277837.com | www.89ut.com | 289650.com | www.306208.com | www.09243.com | 438741.com | www.980590.com | www.266501.com | www.77.me | www.974512.com | www.292947.com | www.135006.cc | www.6587.vip | www.888msc.com | www.759228.com | www.332976.com | www.1647.vip | 402381.com | www.686862.com | www.075631.com | www.4694.com | 088041.com | www.558622.com | www.85221.cc | www.69zr.com | 004705.com | www.342400.com | www.085214.com | 金掌柜www.83993x.com | www.792594.com | www.612009.com | www.033785.com | 500彩票网www.911209.com | www.771264.com | www.114290.com | www.wp61.com | www.997385.com | www.509115.com | www.024611.com | 大赢家彩票平台www.202370.com | www.820922.com | www.112349.com | www.ct0.cc | www.864874.com | www.339582.com | www.172362.com | www.301022.com | www.41306.com | www.4481.cn | 865095.com | 502458.com | www.967047.com | www.661407.com | www.266901.com | www.71sy.cc | 500611.cc | www.731943.com | www.133308.com | www.310825.com | www.4873.biz | 770278.com | www.700093.cc | www.115842.com | www.x22.cm | 776847.com | www.465065.com | www.285122.com | www.1491.me | 959373.com | www.858241.com | www.316278.com | www.062782.com | www.yi80.com | www.952346.com | www.280283.com | www.083282.com | www.25vt.com | 076506.com | www.610019.com | www.312666.com | www.0395.me | 500彩票www.738500.com | www.967026.com | www.277859.com | www.6605.cc | 919004.com | www.841337.com | www.275917.com | www.09570.com | 088094.com | www.788789.cc | www.236778.com | www.5749.cn | 810120.com | www.530641.cc | www.105144.com | 财神网www.29277c.com | www.805998.com | www.249924.com | www.54em.com | 94097.com | www.954842.com | www.357228.com | www.66050.com | www.iw77.com | www.879965.com | www.575840.com | www.046068.com | www.nh24.com | 279903.com | www.355523.com | www.7565.in | 易彩网www.508277.com | 701225.com | www.439400.com | www.062882.com | www.mj16.com | 4235.biz | www.521197.com | www.38246.com | 442797.com | www.632793.com | www.009968.com | www.3758.cm | www.960174.com | www.741239.com | www.860440.com | 964942.com | www.446680.com | www.3953.cc | www.987034.com | www.223337.com | www.9035.top | 367306.com | www.290771.com | www.n24.link | www.778305.com | www.246331.com | www.54iz.com | www.945199.com | www.267638.com | www.b61.top | www.968888.com | www.781564.com | www.517709.com | www.301266.com | www.191717.com | www.xm97.com | www.907578.com | www.210783.com | www.77ce.com | 930697.com | www.514426.com | www.05868.com | www.vp30.com | 731094.com | www.663111.com | www.176507.com | www.8403.vip | 629637.com | www.682419.com | www.020329.com | www.fh78.cc | www.974422.com | www.276957.com | www.53yv.com | 42nt.com | www.366391.com |